A brief introduction to nmn powder | Bontac

A brief introduction to nmn powder | Bontac

 NMN, stands for Beta nicotinamide mononucleotide  with the CAS NO. of 1094-61-7 and chemical formula of C11H15N2O8P , is a substance naturally present in the human body and participates in the synthesis of NAD+ in cells. NAD+ is the most important coenzyme in the human body. It participates in thousands of biocatalytic reactions in the human body and is an essential substance for the human body. For example, metabolism, redox, DNA maintenance and repair, gene stability, epigenetic regulation, etc., all require the participation of NAD+. NMN can be naturally synthesized in the human body and can also be derived from a variety of foods, including broccoli, cabbage, cucumber, edamame, avocado, etc., but the content is quite rare.  NMN powder is a high purity solid manufactured by a special technique. 

NMN is a metabolite called nicotinamide riboside, which is naturally produced in the body. NMN powder is a powdery substance made of NMN that can be used in pharmaceuticals or dietary supplements. It has been studied as a substance that may help improve aging and chronic diseases. Currently, research on NMN is limited, and its long-term effects on humans are unclear.

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Advantages of NMNH

NMNH: 1. “Bonzyme” Whole-enzymatic method, environmental-friendly, no harmful solvent residues manufacturing powder. 2. Bontac is a very first manufacture in the world to produce the NMNH powder on the level of high purity, stability. 3. Exclusive “Bonpure” seven-step purification technology, high purity(up to 99%) and stability of production of NMNH powder 4. Self-owned factories and obtained a number of international certifications to ensure high quality and stable supply of products of NMNH powder 5. Provide one-stop product solution customization service

Advantages of NADH

NADH: 1. Bonzyme whole-enzymatic method, environmental-friendly, no harmful solvent residues 2. Exclusive Bonpure seven-step purification technology, purity up higher than 98 % 3. Special patented process crystal form, higher stability 4. Obtained a number of international certifications to ensure high quality 5. 8 domestic and foreign NADH patents, leading the industry 6. Provide one-stop product solution customization service

Advantages of NAD

NAD:  1. “Bonzyme” Whole-enzymatic method, environmental-friendly, no harmful solvent residues 2. Stable supplier of 1000+ enterprises around the world 3. Unique “Bonpure” seven-step purification technology, higher product content and higher conversion rate 4. Freeze drying technology to ensure stable product quality 5. Unique crystal technology, higher product solubility 6. Self-owned factories and obtained a number of international certifications to ensure high quality and stable supply of products

Advantages of MNM

NMN:  1. “Bonzyme”Whole-enzymatic method, environmental-friendly, no harmful solvent residues 2. Exclusive“Bonpure”seven-step purification technology, high purity(up to 99.9%) and stability 3. Industrial leading technology: 15 domestic and international NMN patents 4. Self-owned factories and obtained a number of international certifications to ensure high quality and stable supply of products 5. Multiple in vivo studies show that Bontac NMN is safe and effective 6. Provide one-stop product solution customization service 7. NMN raw material supplier of famous David Sinclair team of Harvard University

about BONTAC

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Bontac Bio-Engineering (Shenzhen) Co., Ltd. (hereafter referred to as BONTAC) is a high-tech enterprise established in July 2012. BONTAC integrates R&D, production and sales, with enzyme catalysis technology as the core and coenzyme and natural products as main products. There are six major series of products in BONTAC, involving coenzymes, natural products, sugar substitutes, cosmetics, dietary supplements and medical intermediates.

As the leader of the global NMN industry, BONTAC has the first whole-enzyme catalysis technology in China. Our coenzyme products are widely used in health industry, medical & beauty, green agriculture, biomedicine and other fields. BONTAC adheres to independent innovation, with more than 170 invention patents. Different from the traditional chemical synthesis and fermentation industry, BONTAC has advantages of green low-carbon and high-value-added biosynthesis technology. What’s more, BONTAC has established the first coenzyme engineering technology research center at the provincial level in China which also is the sole in Guangdong Province.

In the future, BONTAC will focus on its advantages of green, low-carbon and high-value-added biosynthesis technology, and build ecological relationship with academia as well as upstream/downstream partners, continuously leading the synthetic biological industry and creating a better life for human beings.

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Unlocking the Secrets of NMN: Mechanism and Effects on Health

In the world of cutting-edge biotechnology, few compounds have garnered as much attention as Nicotinamide Mononucleotide, or NMN. This revolutionary molecule is taking the health and wellness industry by storm, and for a good reason. In this article, we will explore what NMN is, its mechanisms, and its profound effects on health.

What is NMN?

Nicotinamide Mononucleotide (NMN) is a naturally occurring compound found in all living cells. It plays a crucial role in the production of Nicotinamide Adenine Dinucleotide (NAD+), a coenzyme that is essential for various biological processes in the body. NAD+ levels tend to decline as we age, contributing to various age-related health issues. NMN supplementation aims to boost NAD+ levels, potentially reversing or mitigating these issues.

The Mechanism: How NMN Works

NMN is a precursor to NAD+, meaning it is converted into NAD+ in our cells. This transformation is vital because NAD+ is involved in several critical cellular functions, including energy metabolism, DNA repair, and gene expression. When NMN is administered, it enhances the NAD+ levels, providing the body with more of this essential coenzyme.

Effects of NMN on Health

The increased availability of NAD+ through NMN supplementation has far-reaching effects on health and wellness:

1、Energy Production: NAD+ is a key player in the production of adenosine triphosphate (ATP), the body's primary energy currency. More NAD+ means more energy for the body's cells, potentially leading to increased vitality and stamina.

2、Cellular Repair: NAD+ supports DNA repair mechanisms, helping to maintain the integrity of the genome. This is crucial in preventing mutations that can lead to aging and the development of diseases.

3、Sirtuin Activation: NAD+ is necessary for the activation of sirtuins, a family of proteins that regulate cellular health. Sirtuins have been associated with longevity and can help combat age-related issues.

4、Metabolic Health: Increased NAD+ levels can have a positive impact on metabolic processes, potentially aiding in weight management and preventing metabolic disorders.

5、Neuroprotection: Some studies suggest that NAD+ augmentation with NMN may help protect against neurodegenerative diseases like Alzheimer's and Parkinson's.

6、Cardiovascular Health: NAD+ plays a role in maintaining healthy blood vessels, which can contribute to cardiovascular health.

7、Immune System Support: NAD+ helps regulate the immune system, potentially enhancing its ability to respond to infections and diseases.

Is NMN Safe?

NMN is generally considered safe for use, and it has been extensively studied in animal models. However, more research is needed to fully understand its long-term effects in humans. As with any supplement, it's crucial to consult with a healthcare professional before incorporating NMN into your health regimen.

In conclusion, NMN is a remarkable compound with the potential to revolutionize health and wellness. By increasing NAD+ levels, NMN supplementation can positively impact energy levels, cellular repair, metabolic health, and more. As the biotechnology industry continues to explore the possibilities of NMN, it may become a key player in the quest for longevity and enhanced well-being.

At Bontac, we are at the forefront of providing high-quality NMN raw materials for businesses in the health and wellness industry. Our commitment to quality and innovation ensures that our partners can harness the full potential of NMN. Contact us to learn more about our NMN offerings and how we can assist your business in delivering cutting-edge products to the market.

NMN: Manufacturing, Preparation, and Clinical Trials

In the realm of cutting-edge biotechnology, one compound has taken center stage for its potential to transform the landscape of health and wellness. Nicotinamide Mononucleotide (NMN) has been the subject of significant research, with a focus on its manufacturing and preparation processes, as well as its clinical trials.

NMN Manufacturing and Preparation

NMN is a derivative of niacin (vitamin B3) and is a precursor to Nicotinamide Adenine Dinucleotide (NAD+), a critical coenzyme involved in various cellular functions. Producing NMN involves several steps to ensure its purity and effectiveness:

1、Extraction: NMN can be synthesized from niacin or obtained through the extraction of natural sources like milk. These sources contain precursors that can be converted into NMN.

2、Purification: After extraction, NMN must undergo purification processes to remove impurities and achieve the desired level of purity. High purity is essential to ensure the efficacy and safety of NMN supplements.

3、Synthesis: Chemical synthesis is another method for manufacturing NMN. This involves converting niacin into NMN through a series of chemical reactions. This method offers precise control over the NMN production process.

4、Quality Control: Throughout the manufacturing process, rigorous quality control measures are applied to guarantee the safety and effectiveness of the final product. These measures include testing for purity, potency, and absence of contaminants.

The manufacturing and preparation of NMN require careful attention to detail to ensure that the end product meets the highest standards for quality and safety.

NMN Clinical Trials

Clinical trials related to NMN have provided valuable insights into its potential benefits. These trials have explored various aspects of NMN supplementation, shedding light on its safety and effectiveness:

1、Aging and Longevity: Some studies have suggested that NMN supplementation may have a positive impact on aging-related issues, potentially extending lifespan and improving overall health.

2、Metabolic Health: Clinical trials have examined the effects of NMN on metabolic health. Preliminary results have indicated potential benefits in areas such as glucose metabolism and insulin sensitivity.

3、Cardiovascular Health: Research has investigated the impact of NMN on cardiovascular health. Promising findings have emerged regarding blood vessel function and blood pressure regulation.

4、Neuroprotection: NMN's potential neuroprotective properties have been studied in the context of neurodegenerative diseases. While more research is needed, early results have been encouraging.

5、Safety and Tolerability: Clinical trials have also focused on the safety and tolerability of NMN. Generally, NMN has been well-tolerated, with few reported side effects.

It's important to note that while these clinical trials have provided valuable data, the research on NMN is ongoing, and more extensive studies are needed to establish its long-term safety and efficacy.

Bontac, a leading biotech enterprise, is at the forefront of providing high-quality NMN raw materials. Our commitment to innovation and quality ensures that our partners can develop products that meet the highest standards in the health and wellness industry. With a strong emphasis on research and development, we aim to contribute to the ongoing exploration of NMN's potential benefits.

In conclusion, NMN manufacturing and preparation involve a series of rigorous steps to ensure the production of high-quality, safe supplements. Clinical trials have provided promising insights into NMN's potential benefits in areas such as aging, metabolic health, cardiovascular health, and neuroprotection. As research on NMN continues, Bontac remains dedicated to providing the industry with the finest NMN raw materials for the development of innovative health and wellness products.

Best NMN Powder: Factory, Purity, and Supplier

When searching for the best NMN (Nicotinamide Mononucleotide) powder, three key aspects play a pivotal role in your decision-making process. This comprehensive guide dives into the world of NMN, highlighting the critical factors that set the best NMN powder apart. We'll explore the best NMN powder factory, delve into the significance of purity, and introduce you to a reliable NMN powder supplier.

  1. Best NMN Powder Factory :
  2. State-of-the-Art Manufacturing:

The foundation of premium NMN powder begins at the factory. Look for a manufacturer that boasts state-of-the-art facilities, adhering to the highest standards of production. Top-tier factories utilize cutting-edge technology and rigorous quality control to ensure the finest NMN powder.

  1. Research and Development:

Leading NMN powder factories invest in robust research and development. They actively engage in scientific studies and collaborate with experts to continuously enhance their product's quality and effectiveness.

  1. Quality Certifications:

Reputable factories hold certifications such as GMP (Good Manufacturing Practices) and ISO, underscoring their commitment to quality, safety, and consistency. These certifications are a testament to their dedication to producing the best NMN powder.

  1. Best NMN Powder Purity (approx. 200 words):
  2. High Purity Standards:

The purity of NMN powder is paramount. Opt for a product with a purity rating of 99% or higher. The best NMN powder is free from impurities and excipients, ensuring maximum potency.

  1. Independent Testing:

To validate purity claims, the best NMN powder undergoes independent third-party testing. These tests provide transparency and assurance that what you're purchasing aligns with its purity claims.

  1. No Additives or Fillers:

A top-quality NMN powder doesn't contain additives or fillers. The absence of unnecessary substances ensures you're receiving a pure and potent product.

  1. Best NMN Powder Supplier:
  2. Reliability and Reputation:

Selecting the right supplier is crucial. Look for a supplier with a stellar reputation for delivering consistent, high-quality NMN powder. Online reviews and testimonials can offer insights into their reliability.

  1. Transparent Sourcing:

A trustworthy supplier should be transparent about the sourcing and production of their NMN powder. Clear information about their supply chain and production process demonstrates their commitment to quality.

  1. Customer Support:

The best NMN powder supplier offers excellent customer support. Whether you have questions or need assistance, a responsive and knowledgeable support team is a valuable asset.

In the quest for the best NMN powder, the factory, purity, and supplier are non-negotiable elements. By opting for a manufacturer with cutting-edge facilities, a commitment to purity, and a reliable supplier with a sterling reputation, you'll embark on a journey towards reaping the full benefits of NMN. The best NMN powder is not just a product; it's a commitment to your well-being.

User Reviews

What users say about BONTAC

BONTAC is a reliable partner that we have been working with for many years. The purity of their coenzyme is very high. Their COA can achieve relatively high test results.

Front

I discovered BONTAC in 2014 because David's article in cell about NAD and NMN related showed that he used BONTAC's NMN for his experimental material. Then we found them in China. After so many years of cooperation, I think it is a very good company.

Hanks

I think green, healthy and high purity are the advantages of BONTAC's products compared with others. I still work with them to this day.

Phillip

In 2017, we chose BONTAC's coenzyme, during which our team encountered many technical problems and consulted their technical team, which were able to give us good solutions. Their products are shipped very fast and they work more efficiently.

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Frequently Asked Question

Do you have any question?

Aging, as a natural process is identified by downregulation of energy production in mitochondria of various organs such as brain, adipose tissue, skin, liver, skeletal muscle and pancreas due to the depletion of NAD+ . NAD+ levels in the body decrease as a consequence of increasing NAD+ consuming enzymes when aging There are three different biosynthesis pathways to produce NAD+ in mammalian cells including de novo synthesis from tryptophan, salt and Preiss-Handler pathways. Among these three pathways, NMN is an interproduct by is involved in NAD+ biosynthesis through salt and Preiss-Handler pathways. The salvage pathway is the most efficient and the main route for the NAD+ biosynthesis, in which nicotinamide and 5-phosphoribosyl-1-pyrophosphate are converted to NMN with the enzyme of NAMPT followed by conjugation to ATP and conversion to NAD by NMNAT. Furthermore, NAD+ consuming enzymes are responsible for degradation of NAD+ and consequence nt formation of nicotinamide as a by-product.

The safety of NMN powder cannot be assessed since required clinical and toxicological studies have not been completed yet to establish the recommended safe levels for long term administration. Nevertheless, their safety and efficacy are uncertain and unreliable since most of them have not been back by Rigorous scientific preclinical and clinical testing. This issue has been arisen as manufacturers are hesitant to pay for research and clinical trials due to potential lower profit margin, and there is no authorizing agency to regulate NMN products because it is often product sold as functional food than heavily regulated therapeutic drug. Therefore, more strict approval process has been demanded by consumer advocacy groups requesting regulatory agencies to set standard and restrictions for marketing anti-aging health products, considering safety, health and wellbeing of N red besumers. a panacea for the elderly, because boosting NAD levels when not required may yield some detrimental effects. Therefore, the dose and frequency of NMN supplementation should be carefully prescribed depending on the type of age-related deficiency and all other confronting health conditions of the people. Other NAD precursors over have been studied to diverse age-related deficiencies and they are used for particular deficiencies, only after they are proven for effectiveness and safe to use. Therefore, the same principle should be applied to NMN as well

First, inspect the factory. After some screening, NMN companied that directly face consumers pay more attention to brand building. Therefore, for a good brand, quality is the most important thing, and the first thing to control the quality of raw materials is to inspect the factory. Bontac company actually manufacturing NMN powder of high quality with the caterias of SGS. Secondly, the purity is tested. Purity is one of the most important parameters of NMN powder. If high purity NMN cannot be guaranteed, the remaining substances are likely to exceed the relevant standards. As the attached certificates demonstrates that the NMN powder produced by Bontac reach the purity of 99.9%. Finally, a professional test spectrum is needed to prove it. Magnetic Resonance Spectroscopy (NMR) and high-resolution mass spectrometry (HRMS). Usually through the analysis of these two spectra, the structure of the compound ca n be preliminarily determined.

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Unraveling Impacts of Ginsenoside Rg3 Treatment on IL-1β-induced Injury of NPCs

Introduction Intervertebral disc degeneration (IDD) is a frequently seen orthopedic disease, which is accompanied with excessive apoptosis of nucleus pulposus cells (NPCs) and degeneration of extracellular matrix (ECM), with main symptoms of pain and numbness in the waist, legs and feet, as well as inflammation on and around the surface of bone tissues. Strikingly, ginsenoside Rg3, the main active ingredient of ginseng, has been attested to exhibit anti-catabolic and anti-apoptotic effects in IL-1β-treated human NPCs and IDD rats by inactivating the p38 MAPK pathway. The risk factors for IDD IDD is generally associated with risk factors such as aging, excessive exercise, working environment, and genetics. As one ages, the amount of water in the body and in the intervertebral discs will be reduced accordingly. Intervertebral discs that lack moisture will lose their elastic function and become hard. Once there is any stimulation or pressure, the intervertebral disc may crack, leading to intervertebral disc injury. For instance, the mechanical trauma caused by excessive exercise and work may accelerate the fragility of disc and exacerbate IDD. Anti-catabolic and anti-apoptotic effects of ginsenoside Rg3 in IL-1β-treated human NPCs and IDD rats Ginsenoside Rg3 plays an anti-apoptotic role in IL-1β-treated human NPCs and IDD rats, as evidenced by the down-regulation of pro-apoptosis protein Bax and up-regulation of anti-apoptosis protein Bcl-2 in IL-1β-stimulated NPCs and IDD model rats. Besides, ginsenoside Rg3 represses ECM degradation in IL-1β-stimulated NPCs and intervertebral disc tissues of IDD rats, as attested by the decreased expression of ECM degradation-related factors MMPs (MMP2 and MMP3) and ADAMTSs (Adamts4, and Adamts5). Ginsenoside Rg3 exhibits anti-catabolic and anti-apoptotic effects in IL-1β-treated human NPCs. Ginsenoside Rg3 reduces apoptosis and catabolism in IDD rats. Alleviation of ginsenoside Rg3 in IDD via p38 MAPK pathway Ginsenoside Rg3 can alleviate NPC degeneration, recover the arrangement of annulus fibrous, and preserve more proteoglycan matrix via inactivating p38 MAPK pathway. In vitro, the fluorescence intensity of p38 is enhanced in IL-1β-stimulated NPCs, yet ginsenoside Rg3 offsets this promoting effect. In vivo, the phosphorylated p38 level is elevated in NPCs and the intervertebral disc tissues of IDD rats, while ginsenoside Rg3 works inversely. Ginsenoside Rg3 suppresses the IL-1β-stimulated p38 MAPK pathway in human NPCs Ginsenoside Rg3 inactivates the p38 MAPK pathway in IDD rats. Conclusion The anti-catabolic and anti-apoptotic effects of ginsenoside Rg3 in IL-1β treated human disc nucleus pulposus cells and in a rat model of disc degeneration are accomplished by inactivating the MAPK pathway, providing new clues on the treatment of IDD. Reference Chen J, Zhang B, Wu L, et al. Ginsenoside Rg3 exhibits anti-catabolic and anti-apoptotic effects in IL-1β treated human disc nucleus pulposus cells and in a rat model of disc degeneration by inactivating the MAPK pathway. Cell Mol Biol. 2024;70(1):233-238. doi:10.14715/cmb/2024.70.1.32 BONTAC Ginsenosides BONTAC has been dedicated to the R&D, manufacture and sale of raw materials for coenzyme and natural products since 2012, with self-owned factories, over 170 global patents as well as strong R&D team. BONTAC has rich R&D experience and advanced technology in the biosynthesis of rare ginsenosides Rh2/Rg3, with pure raw materials, higher conversion rate and higher content (up to 99%). One-stop service for customized product solution is available in BONTAC. With unique Bonzyme enzymatic synthesis technology, both S-type and R-type isomers can be accurately synthesized here, with stronger activity and precise targeting action. Our products are subjected to strict third-party self-inspection, which are worth of trustworthy. Disclaimer This article is based on the reference in the academic journal. The relevant information is provided for sharing and learning purposes only, and does not represent any medical advice purposes. If there is any infringement, please contact the author for deletion. The views expressed in this article do not represent the position of BONTAC. Under no circumstances will BONTAC be responsible or liable in any way for any claims, damages, losses, expenses or costs  resulting or arising directly or indirectly from your reliance on the information and material on this website.

Targeting NAD+ Salvage Pathway as a Potential Approach to Combat Obesity

Introduction Mar 4th is determined as the World Obesity Day. World Obesity Federation, UNICEF and WHO have hosted a global youth-led webinar to talk about obesity & youth. The obesity crisis has gradually attracted much attention. The latest report by the Lancet suggests that one billion people are bothered by obesity (2022), with 650 million adults, 340 million adolescents and 39 million children. Recently, etiological studies and interventions for obesity have been progressively focused on the central nervous system, with an attempt to curb the onset of obesity at its source. Notably, targeting NAD+ salvage pathway in hypothalamic astrocytes may be a potential approach to combat obesity. The association of hypothalamic astrocytes and obesity The hypothalamus functions as the appetite regulation center, which receives and integrates the neuroendocrine factors produced by the central nervous system and peripheral tissues to promote or suppress appetite, so as to affect body weight. Noteworthily, aypothalamic astrocytes can apparently decrease glucose clearance and increase plasma insulin levels, playing an essential role in modulating energy metabolism, which are expected to be a new target for obesity treatment. Alleviation of high-fat diet (HFD)-induced obesity by repressing astrocyte NAD+ salvage pathway Under conditions of excessive fat intake, the NAD+ salvage pathway is specifically activated in hypothalamic astrocytes, which restrains the energy expenditure (EE) and fat oxidation in adipose tissues by downregulating sympathetic nerve innervation, eventually resulting in the accumulation of adipose tissue fat and the development of obesity. CD38 as a downstream mediator of astrocyte inflammation induced by the NAD+ salvage pathway. CD38 functions downstream of the NAD+ salvage pathway in hypothalamic astrocytes burdened with excess fat. CD38 knockdown in arcuate nucleus astrocytes diminishes the weight gain, reduces fat mass, increases EE, and lowers RER during HFD consumption. Cd38 depletion in hypothalamic astrocytes may improve hypothalamic inflammation by increasing NAD+ level. Hypothalamic inflammation can not only lead to energy imbalances, but also exacerbate central insulin resistance and leptin resistance, which can lead to the accumulation of fat in peripheral tissues. The role of nicotinamide phosphoribosyltransferase (NAMPT)–NAD+–CD38 axis in obesity In mammals, the salvage pathway represents the primary means of maintaining cellular NAD+ level. A crucial step in the NAD+ salvage pathway is catalyzed by NAMPT. In response to fat overload, the activation of the astrocytic NAMPT-NAD+-CD38 axis induces pro-inflammatory responses in the hypothalamus, eliciting aberrantly activated basal Ca2+ signals and compromised Ca2+ responses to metabolic hormones such as insulin, leptin, and glucagon-like peptide 1, ultimately resulting in dysfunctional hypothalamic astrocytes and contribute to the development of obesity. Conclusion Mechanically, inhibition of hypothalamic astrocytic NAD+ salvage pathway, along with its downstream CD38, mitigates hypothalamic inflammation and attenuates the development of HFD-induced obesity in male mice. Reference Park, J.W., Park, S.E., Koh, W. et al  (2024). Hypothalamic astrocyte NAD+ salvage pathway mediates the coupling of dietary fat overconsumption in a mouse model of obesity. Nat Commun 15, 2102. https://doi.org/10.1038/s41467-024-46009-0 BONTAC NAD BONTAC has been dedicated to the R&D, manufacture and sale of raw materials for coenzyme and natural products since 2012, with self-owned factories, over 170 global patents as well as strong R&D team. BONTAC has rich R&D experience and advanced technology in the biosynthesis of NAD and its precursors (eg. NMN and NR). There are various types of NAD to be selected, encompassing NAD ER Grade (endoxin removal), NAD Grade I (IVD/dietary supplement/cosmetics raw powder), NAD Grade II (API/intermediates) and NAD Grade IV (if any higher requirement on the solubility), which can be provided in the form of lyophilized powder or crystalline powder. The purity of BONTAC NAD can reach above 98%.  Disclaimer This article is based on the reference in the academic journal. The relevant information is provided for sharing and learning purposes only, and does not represent any medical advice purposes. If there is any infringement, please contact the author for deletion. The views expressed in this article do not represent the position of BONTAC. BONTAC holds no responsibility for any claims, damages, losses, expenses or costs resulting or arising directly or indirectly from your reliance on the information and material on this website.

The Molecular Mechanisms Underlying the Interaction Between NAD+/NMN and DBC1

Introduction Oxidized form of nicotinamide adenine dinucleotide (NAD+) and its precursor nicotinamide mononucleotide (NMN) have been uncovered to restore DNA repair and prevent cancer progression via the deleted in breast cancer 1 (DBC1). This research is committed to deciphering the detailed molecular mechanisms. About DBC1 DBC1 is a nuclear protein initially cloned from a human chromosome 8p21 region, which can modulate diversified targets by protein-protein interaction, contributing to various cellular processes such as apoptosis, DNA repair, senescence, transcription, metabolism, circadian cycle, epigenetic regulation, cell proliferation, and tumorigenesis. The affinity and molecular binding mechanisms between NAD+/NMN and DBC1354–396 Under the help of nuclear magnetic resonance (NMR) and Isothermal titration calorimetry (ITC) experiments, it is verified that both NAD+ and NMN have a binding relationship with the NHD domain of DBC1. Specifically, NAD+ interacts with DBC1354-396 through hydrogen bonds, with a binding affinity (8.99 μM) nearly twice that of NMN (17.0 μM) and the key binding sites are primarily residues E363 and D372. The vital roles of E363 and D372 mutagenesis in ligand-protein interaction The N-terminal loop of DBC1354-396 encloses the small ligand within a local space, anchoring NAD+ and NMN to the protein through key amino acid residues E363 and D372 via hydrogen bonding. Conclusion Both NAD+ and its precursor NMN can bind to DBC1's NHD domain (DBC1354–396) at key sites E363 and D372, providing novel clues for the development of targeted therapies and drug research on DBC1-associated disease including tumors. Reference Ou L, Zhao X, Wu IJ, et al. Molecular mechanism of NAD+ and NMN binding to the Nudix homology domains of DBC1. Int J Biol Macromol. Published online February 12, 2024. doi:10.1016/j.ijbiomac.2024.130131 BONTAC NAD BONTAC has been dedicated to the R&D, manufacture and sale of raw materials for coenzyme and natural products since 2012, with self-owned factories, over 170 global patents as well as strong R&D team consisting of Doctors and Masters. BONTAC has rich R&D experience and advanced technology in the biosynthesis of NAD and its precursors (eg. NMN), with various forms to be selected (eg. endoxin-free IVD-grade NAD, Na-free or Na-containing NAD; NR-CL or NR-Malate). High quality and stable supply of products can be better ensured here with the exclusive Bonpure seven-step purification technology and Bonzyme Whole-enzymatic method. Disclaimer This article is based on the reference in the academic journal. The relevant information is provided for sharing and learning purposes only, and does not represent any medical advice purposes. If there is any infringement, please contact the author for deletion. The views expressed in this article do not represent the position of BONTAC. Under no circumstances will BONTAC be held responsible or liable in any way for any claims, damages, losses, expenses, costs or liabilities whatsoever (including, without limitation, any direct or indirect damages for loss of profits, business interruption or loss of information) resulting or arising directly or indirectly from your reliance on the information and material on this website.

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